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The improvement of riboflavin production in Ashbya gossypii via disparity mutagenesis and DNA microarray analysis
http://hdl.handle.net/10297/5802
http://hdl.handle.net/10297/5802717aba57-2db0-49c7-871d-86a402e18464
名前 / ファイル | ライセンス | アクション |
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110811003.pdf (1.3 MB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||
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公開日 | 2011-08-12 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | The improvement of riboflavin production in Ashbya gossypii via disparity mutagenesis and DNA microarray analysis | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Park, Enoch Y.
× Park, Enoch Y.
WEKO
2907
× Ito, Yoko× Nariyama, Masashi× Sugimoto, Takashi× Lies, Dwiarti× Kato, Tatsuya
WEKO
2910
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書誌情報 |
Applied Microbiology and Biotechnology 巻 91, 号 5, p. 1315-1326, 発行日 2011-09 |
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出版者 | ||||||||||
出版者 | Springer | |||||||||
権利情報 | ||||||||||
権利情報 | Copyright © 2011, Springer-Verlag | |||||||||
NDC | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 464 | |||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | We generated a high riboflavin-producing mutant strain of Ashbya gossypii by disparity mutagenesis using mutation of DNA polymerase δ in the lagging strand, resulting in loss of DNA repair function by the polymerase. Among 1,353 colonies generated in the first screen, 26 mutants produced more than 3 g/L of riboflavin. By the second screen and single-colony isolation, nine strains that produced more than 5.2 g/L of riboflavin were selected as high riboflavin-producing strains. These mutants were resistant to oxalic acid and hydrogen peroxide as antimetabolites. One strain (W122032) produced 13.7 g/L of riboflavin in a 3-L fermentor using an optimized medium. This represents a ninefold improvement on the production of the wild-type strain. Proteomic analysis revealed that ADE1, RIB1, and RIB5 proteins were expressed at twofold higher levels in this strain than in the wild type. DNA microarray analysis showed that purine and riboflavin biosynthetic pathways were upregulated, while pathways related to carbon source assimilation, energy generation, and glycolysis were downregulated. Genes in the riboflavin biosynthetic pathway were significantly overexpressed during both riboflavin production and stationary phases, for example, RIB1 and RIB3 were expressed at greater than sixfold higher levels in this strain compared to the wild type. These results indicate that the improved riboflavin production in this strain is related to a shift in carbon flux from β-oxidation to the riboflavin biosynthetic pathway. | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 01757598 | |||||||||
EISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 14320614 | |||||||||
NII書誌ID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10503386 | |||||||||
PubMed番号 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | PMID | |||||||||
関連識別子 | 21573938 | |||||||||
出版者DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | 10.1007/s00253-011-3325-0 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |