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A survey of proteases in edible mushrooms with synthetic peptides as substrates
http://hdl.handle.net/10297/5854
http://hdl.handle.net/10297/5854e62eed9a-f113-42a7-9a3a-dffd21609e1d
名前 / ファイル | ライセンス | アクション |
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110823001.pdf (316.8 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||
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公開日 | 2011-08-23 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | A survey of proteases in edible mushrooms with synthetic peptides as substrates | |||||||
言語 | ||||||||
言語 | eng | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Nakamura, Mayumi
× Nakamura, Mayumi× Iketani, Aya× Shioi, Yuzo
WEKO
3078
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書誌情報 |
Mycoscience 巻 52, 号 4, p. 234-241, 発行日 2011-07 |
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出版者 | ||||||||
出版者 | Springer | |||||||
権利情報 | ||||||||
権利情報 | The original publication is available at www.springerlink.com | |||||||
NDC | ||||||||
主題Scheme | NDC | |||||||
主題 | 464 | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | The protease activities in six edible mushrooms were surveyed using synthetic fluorogenic substrates that have different specificities for each protease group. The activity was determined by measuring the fluorogenic intensity of the 7-amino-4-methylcoumarin (AMC) liberated by an enzyme. Various types of activities were found in all mushrooms, and their activities depended largely on the mushroom species, but also on the pH and localization. Flammulina velutipes and Pleurotus eryngii had the widest and highest proteolytic activities among the six mushrooms examined. The proteasome-like protease activities were generally much higher than those of other proteases. High caspase activities, which occur during apoptosis in cells, were detected in two mushrooms, F. velutipes and Hypsizigus marmoreus. The pH optima of the proteolytic activities were largely divided into two groups, acidic pH 5–6 for caspases and neutral to alkaline (pH 6.5–11) for the others. In F. velutipes, higher proteolytic activity was observed in the basement of the stem than in the cap and stem. Purification and characterization of protease were also carried out to identify a protease from Grifola frondosa using t-butyloxycarbonyl-Leu-Arg-Arg-4-methylcoumaryl-7-amide (Boc-LRR-MCA) as the substrate. | |||||||
ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 13403540 | |||||||
EISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 16182545 | |||||||
NII書誌ID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AA11003436 | |||||||
出版者DOI | ||||||||
関連タイプ | isVersionOf | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | 10.1007/s10267-010-0089-9 | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | AM | |||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |