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Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure
http://hdl.handle.net/10297/5175
http://hdl.handle.net/10297/5175e401d3ff-58d6-409c-854f-ce49ef7e6e79
名前 / ファイル | ライセンス | アクション |
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100702005.pdf (569.2 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||
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公開日 | 2010-07-02 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Animals | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Blotting, Western | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Bombyx | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Carbohydrate Conformation | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Cloning, Molecular | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Hemolymph | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Humans | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Immunoglobulin G | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Larva | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Lectins | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Nucleopolyhedrovirus | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Polysaccharides | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Recombinant Proteins | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Park, Enoch Y.
× Park, Enoch Y.
WEKO
2907
× Ishikiriyama, Motoki× Nishina, Takuya× Kato, Tatsuya
WEKO
2910
× Yagi, Hirokazu× Kato, Koichi× Ueda, Hiroshi |
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書誌情報 |
Journal of Biotechnology 巻 139, 号 1, p. 108-114, 発行日 2009-01-01 |
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出版者 | ||||||||||
出版者 | Elsevier | |||||||||
権利情報 | ||||||||||
権利情報 | Copyright © 2008 Elsevier B.V. All rights reserved. | |||||||||
NDC | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 465 | |||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 IgG was constructed and used to secrete recombinant antibody into silkworm larval hemolymph. Fifth instar silkworm larvae were reared and injected into the dorsum of the larvae with recombinant cysteine protease- and chitinase-deficient BmNPV (BmNPV-CP(-)-Chi(-)) bacmid/29IJ6 IgG and harvested after approximately 6 days. The total yield of recombinant 29IJ6 IgG was 36 microg/larvae, which is equivalent to 8 mg/kg of larvae. The recombinant antibody was purified to homogeneity using a HiTrap rProtein A FF column with a purification yield of 83.1%. The purified protein was identified by Western blot and ELISA experiments. The N-linked glycan structure of the purified protein was determined by the HPLC mapping method. The N-glycans of the 29IJ6 IgG glycoprotein produced in, and secreted by the silkworm larvae were composed exclusively of two kinds of paucimannose-type oligosaccharides, Manalpha1-6Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc and Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc. | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 01681656 | |||||||||
NII書誌ID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10458361 | |||||||||
PubMed番号 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | PMID | |||||||||
関連識別子 | 18984019 | |||||||||
出版者DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | 10.1016/j.jbiotec.2008.09.013 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
出版者版 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | URI | |||||||||
関連識別子 | https://doi.org/10.1016/j.jbiotec.2008.09.013 |