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Flow cytometry-based method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl.
http://hdl.handle.net/10297/5565
http://hdl.handle.net/10297/556530f5e15b-e658-48e0-bd77-1fe67a36e49c
名前 / ファイル | ライセンス | アクション |
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110310001.pdf (583.0 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||
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公開日 | 2011-03-10 | |||||||
タイトル | ||||||||
言語 | en | |||||||
タイトル | Flow cytometry-based method for isolating live bacteria with meta-cleavage activity on dihydroxy compounds of biphenyl. | |||||||
言語 | ||||||||
言語 | eng | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Animals | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Bacteria | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Biphenyl Compounds | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Comamonas testosteroni | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Dioxygenases | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Fatty Acids, Unsaturated | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Flow Cytometry | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Fluorescence | |||||||
キーワード | ||||||||
言語 | en | |||||||
主題Scheme | Other | |||||||
主題 | Polychlorinated Biphenyls | |||||||
資源タイプ | ||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||
資源タイプ | journal article | |||||||
著者 |
Iijima, Sou
× Iijima, Sou× Shimomura, Yumi× Haba, Yousuke× Kawai, Fusako× Tani, Akio× Kimbara, Kazuhide
WEKO
4890
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書誌情報 |
Journal of Bioscience and Bioengineering 巻 109, 号 6, p. 645-651, 発行日 2010-06 |
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出版者 | ||||||||
出版者 | Elsevier | |||||||
権利情報 | ||||||||
権利情報 | Copyright © 2009 Published by Elsevier B.V. | |||||||
NDC | ||||||||
主題Scheme | NDC | |||||||
主題 | 465 | |||||||
抄録 | ||||||||
内容記述タイプ | Abstract | |||||||
内容記述 | A new method for isolating targeted live bacterial cells was established with the use of cell sorting by flow cytometry (FCM) based on the fluorescence of the intermediate metabolite of biphenyl degradation. During biphenyl degradation, a PCB degrader, Comamonas testosteroni TK102, produces a meta-cleavage intermediate metabolite, 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoic acid (HOPDA), which emits green fluorescence. HOPDA was produced from 2,3-dihydroxy biphenyl as a substrate, but it was not appropriate for labeling cells because it was released from the cells into the medium. When we used 4-n-butylbiphenyl and 4-n-heptylbiphenyl, we found that the cells produced and accumulated 2,3-dihydroxy intermediate metabolites. By the addition of synthesized 2,3-dihydroxy-4'-butylbiphenyl (2,3-DHBBP), we were able to label the cells with strong green fluorescence, suggesting the persistence of fluorescent intermediate metabolite in the cells by the introduction of the alkyl tail. 2,3-DHBBP was then used to label strain TK102 and the cells were sorted with FCM. The sorting efficiency of FCM was defined as the percentage of colony numbers per sorting events. Strain TK102 cells were successfully enriched by 4.1-fold from the mixture with environmental indigenous bacteria with a sorting efficiency of 7.3%. The method we present here serves as a basic technique for the specific and direct isolation of live bacterial cells which contain dioxygenases active on dihydroxylated aromatic compounds. | |||||||
ISSN | ||||||||
収録物識別子タイプ | ISSN | |||||||
収録物識別子 | 13474421 | |||||||
NII論文ID | ||||||||
関連タイプ | isVersionOf | |||||||
識別子タイプ | NAID | |||||||
関連識別子 | 110007641915 | |||||||
NII書誌ID | ||||||||
収録物識別子タイプ | NCID | |||||||
収録物識別子 | AA11644703 | |||||||
PubMed番号 | ||||||||
関連タイプ | isVersionOf | |||||||
識別子タイプ | PMID | |||||||
関連識別子 | 20471608 | |||||||
出版者DOI | ||||||||
関連タイプ | isVersionOf | |||||||
識別子タイプ | DOI | |||||||
関連識別子 | 10.1016/j.jbiosc.2009.11.023 | |||||||
フォーマット | ||||||||
内容記述タイプ | Other | |||||||
内容記述 | application/pdf | |||||||
著者版フラグ | ||||||||
出版タイプ | AM | |||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||
出版者版 | ||||||||
関連タイプ | isVersionOf | |||||||
識別子タイプ | URI | |||||||
関連識別子 | https://doi.org/10.1016/j.jbiosc.2009.11.023 |