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Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624
名前 / ファイル | ライセンス | アクション |
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110517001.pdf (127.9 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||||
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公開日 | 2011-05-17 | |||||||||||
タイトル | ||||||||||||
言語 | en | |||||||||||
タイトル | Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624 | |||||||||||
言語 | ||||||||||||
言語 | eng | |||||||||||
資源タイプ | ||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||
資源タイプ | journal article | |||||||||||
著者 |
Sugiura, Tatsuki
× Sugiura, Tatsuki× Yamagishi, Kenji× Kimura, Toshiyuki× Nishida, Tomoaki
WEKO
2665
× Kawagishi, Hirokazu
WEKO
1503
× Hirai, Hirofumi
WEKO
2669
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書誌情報 |
Bioscience, Biotechnology, and Biochemistry 巻 73, 号 8, p. 1793-1798, 発行日 2009 |
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出版者 | ||||||||||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||||||||
NDC | ||||||||||||
主題Scheme | NDC | |||||||||||
主題 | 658 | |||||||||||
抄録 | ||||||||||||
内容記述タイプ | Abstract | |||||||||||
内容記述 | Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful. | |||||||||||
ISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 09168451 | |||||||||||
EISSN | ||||||||||||
収録物識別子タイプ | ISSN | |||||||||||
収録物識別子 | 13476947 | |||||||||||
NII論文ID | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | NAID | |||||||||||
関連識別子 | 10027544518 | |||||||||||
NII書誌ID | ||||||||||||
収録物識別子タイプ | NCID | |||||||||||
収録物識別子 | AA10824164 | |||||||||||
PubMed番号 | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | PMID | |||||||||||
関連識別子 | 19661691 | |||||||||||
出版者DOI | ||||||||||||
関連タイプ | isIdenticalTo | |||||||||||
識別子タイプ | DOI | |||||||||||
関連識別子 | 10.1271/bbb.90152 | |||||||||||
フォーマット | ||||||||||||
内容記述タイプ | Other | |||||||||||
内容記述 | application/pdf | |||||||||||
著者版フラグ | ||||||||||||
出版タイプ | VoR | |||||||||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 |