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Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol
名前 / ファイル | ライセンス | アクション |
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110530001.pdf (415.8 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||||||
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公開日 | 2011-05-30 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Functional characterization of rose phenylacetaldehyde reductase (PAR), an enzyme involved in the biosynthesis of the scent compound 2-phenylethanol | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Alcohol Oxidoreductases | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Amino Acid Sequence | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Phenylethyl Alcohol | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Plant Proteins | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Rosa | |||||||||||||
キーワード | ||||||||||||||
言語 | en | |||||||||||||
主題Scheme | Other | |||||||||||||
主題 | Substrate Specificity | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Chen, Xiao-Min
× Chen, Xiao-Min× Kobayashi, Hiromi× Sakai, Miwa× Hirata, Hiroshi× Asai, Tatsuo
WEKO
1676
× Ohnishi, Toshiyuki
WEKO
4884
× Susanne, Baldermann
WEKO
2944
× Watanabe, Naoharu
WEKO
1672
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書誌情報 |
Journal of Plant Physiology 巻 168, 号 2, p. 88-95, 発行日 2011-01-15 |
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出版者 | ||||||||||||||
出版者 | Elsevier | |||||||||||||
権利情報 | ||||||||||||||
権利情報 | Copyright © 2010 Elsevier GmbH All rights reserved. | |||||||||||||
NDC | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 471 | |||||||||||||
抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | 2-Phenylethanol (2PE) is a prominent scent compound released from flowers of Damask roses (Rosa×damascena) and some hybrid roses (Rosa 'Hoh-Jun' and Rosa 'Yves Piaget'). 2PE is biosynthesized from l-phenylalanine (l-Phe) via the intermediate phenylacetaldehyde (PAld) by two key enzymes, aromatic amino acid decarboxylase (AADC) and phenylacetaldehyde reductase (PAR). Here we describe substrate specificity and cofactor preference in addition to molecular characterization of rose-PAR and recombinant PAR from R.×damascena. The deduced amino acid sequence of the full-length cDNA encoded a protein exhibiting 77% and 75% identity with Solanum lycopersicum PAR1 and 2, respectively. The transcripts of PAR were higher in petals than calyxes and leaves and peaking at the unfurling stage 4. Recombinant PAR and rose-PAR catalyzed reduction of PAld to 2PE using NADPH as the preferred cofactor. Reductase activity of rose-PAR and recombinant PAR were higher for aromatic and aliphatic aldehydes than for keto-carbonyl groups. Both PARs showed that (S)-[4-(2)H] NADPH was preferentially used over the (R)-[4-(2)H] isomer to give [1-(2)H]-2PE from PAld, indicating that PAR can be classified as short-chain dehydrogenase reductase (SDR). | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 01761617 | |||||||||||||
NII書誌ID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA10522948 | |||||||||||||
PubMed番号 | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | PMID | |||||||||||||
関連識別子 | 20650544 | |||||||||||||
出版者DOI | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | 10.1016/j.jplph.2010.06.011 | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||||||
出版者版 | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | URI | |||||||||||||
関連識別子 | https://doi.org/10.1016/j.jplph.2010.06.011 |