WEKO3
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Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae
名前 / ファイル | ライセンス | アクション |
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110811001.pdf (701.1 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||||||
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公開日 | 2011-08-11 | |||||||||||||
タイトル | ||||||||||||||
言語 | en | |||||||||||||
タイトル | Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae | |||||||||||||
言語 | ||||||||||||||
言語 | eng | |||||||||||||
資源タイプ | ||||||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||||||
資源タイプ | journal article | |||||||||||||
著者 |
Deo, Vipin Kumar
× Deo, Vipin Kumar× Tsuji, Yoshitaka× Yasuda, Tomomi× Kato, Tatsuya
WEKO
2910
× Sakamoto, Naonori
WEKO
3113
× Suzuki, Hisao
WEKO
3109
× Park, Enoch Y.
WEKO
2907
|
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書誌情報 |
Journal of Virological Methods 巻 177, 号 2, p. 147-152, 発行日 2011-11 |
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出版者 | ||||||||||||||
出版者 | Elsevier | |||||||||||||
権利情報 | ||||||||||||||
権利情報 | Copyright © 2011 Published by Elsevier B.V. | |||||||||||||
NDC | ||||||||||||||
主題Scheme | NDC | |||||||||||||
主題 | 464 | |||||||||||||
抄録 | ||||||||||||||
内容記述タイプ | Abstract | |||||||||||||
内容記述 | Rous sarcoma virus group antigen protein-based virus-like particles (VLPs) are well known for their structural integrity and ease of handling. VLPs play an important role in drug delivery systems because they can be manipulated with ease. In this study, a new method was established for expressing Rous sarcoma virus group antigen protein based VLPs in silkworm larvae and establishing stably expressing insect cell lines. These VLPs have been isolated by ultracentrifugation using a sucrose step gradient of 10-60% (v/v), and their spherical structure has been confirmed using transmission electron microscopy (TEM). The spherical morphology is similar in both the silkworm larvae and in stably expressing cell lines. Silkworm larvae are better suited for producing Rous sarcoma virus group antigen protein-based VLPs on a large scale; yields from silkworm larvae were approximately 8.2-fold higher than yields from stable cell lines. These VLPs provide a new method for large-scale application in vaccine development and drug delivery systems. | |||||||||||||
ISSN | ||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 01660934 | |||||||||||||
EISSN | ||||||||||||||
収録物識別子タイプ | ISSN | |||||||||||||
収録物識別子 | 18790984 | |||||||||||||
NII書誌ID | ||||||||||||||
収録物識別子タイプ | NCID | |||||||||||||
収録物識別子 | AA00259584 | |||||||||||||
PubMed番号 | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | PMID | |||||||||||||
関連識別子 | 21816175 | |||||||||||||
出版者DOI | ||||||||||||||
関連タイプ | isVersionOf | |||||||||||||
識別子タイプ | DOI | |||||||||||||
関連識別子 | 10.1016/j.jviromet.2011.07.012 | |||||||||||||
フォーマット | ||||||||||||||
内容記述タイプ | Other | |||||||||||||
内容記述 | application/pdf | |||||||||||||
著者版フラグ | ||||||||||||||
出版タイプ | AM | |||||||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |