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Production of Rous sarcoma virus-like particles displaying human transmembrane protein in silkworm larvae and its application to ligand-receptor binding assay
http://hdl.handle.net/10297/5801
http://hdl.handle.net/10297/58013e4f54b3-cf40-4d08-bef6-905206d5d2fa
名前 / ファイル | ライセンス | アクション |
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110811002.pdf (519.6 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||
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公開日 | 2011-08-12 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Production of Rous sarcoma virus-like particles displaying human transmembrane protein in silkworm larvae and its application to ligand-receptor binding assay | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Tsuji, Yoshitaka
× Tsuji, Yoshitaka× Deo, Vipin Kumar× Kato, Tatsuya
WEKO
2910
× Park, Enoch Y.
WEKO
2907
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書誌情報 |
Journal of Biotechnology 巻 155, 号 2, p. 185-192, 発行日 2011-09-10 |
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出版者 | ||||||||||
出版者 | Elsevier | |||||||||
権利情報 | ||||||||||
権利情報 | Copyright © 2011 Elsevier B.V. All rights reserved. | |||||||||
NDC | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 464 | |||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Two types of Rous sarcoma virus (RSV) group-antigen protein (Gag) virus like particles (VLPs), full-length Gag (Gag701) and RSV protease domain (PR)-deleted mutant (Gag577) were expressed in silkworm larvae. Gag577 was secreted into hemolymph efficiently using wild type bacmid (WT), cysteine protease-deficient bacmid (CP(-)), cysteine protease and chitinase-deficient bacmid (CP(-)Chi(-)) bacmids, but comparatively Gag701 secretion levels were low. VLPs were purified on 10-60% (v/v) sucrose density gradient by ultracentrifugation and their structures confirmed under electron microscope. When hPRR and RSV Gag577 were co-expressed in silkworm larvae, human prorenin receptor (hPRR) was displayed on the surface of RSV VLPs, which was detected by Western blotting and immunoelectron microscopy. Moreover, binding of hPRR localized on the surface of VLPs to human prorenin was confirmed by ELISA. These results indicate that active hPRR was displayed on the surface of RSV VLPs, which can be utilized for drug discovery of hPRR blockers to prevent nephropathy. Moreover, this transmembrane protein display system using RSV Gag in silkworm larvae is applicable to expression of intact transmembrane proteins and binding assay of transmembrane proteins to its ligands, especially for transmembrane proteins which cannot be purified from membrane fractions in active states. | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 01681656 | |||||||||
EISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 18734863 | |||||||||
NII書誌ID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA10458361 | |||||||||
PubMed番号 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | PMID | |||||||||
関連識別子 | 21794838 | |||||||||
出版者DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | 10.1016/j.jbiotec.2011.07.008 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa | |||||||||
出版者版 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | URI | |||||||||
関連識別子 | https://doi.org/10.1016/j.jbiotec.2011.07.008 |