{"created":"2023-07-25T10:33:17.773667+00:00","id":4667,"links":{},"metadata":{"_buckets":{"deposit":"d0721b3b-05ea-40cb-9ee0-cc21dc080e43"},"_deposit":{"created_by":3,"id":"4667","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4667"},"status":"published"},"_oai":{"id":"oai:shizuoka.repo.nii.ac.jp:00004667","sets":["80:222"]},"author_link":["1525","4514","1677","2427","4515","4516","4511","4507","1503","1524","4510","4509","4513","4512","3235"],"item_26_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2010-04","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"5-6","bibliographicPageEnd":"657","bibliographicPageStart":"648","bibliographicVolumeNumber":"71","bibliographic_titles":[{"bibliographic_title":"Phytochemistry"}]}]},"item_26_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_26_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Ingestion of the toxic mushroom Boletus venenatus causes a severe gastrointestinal syndrome, such as nausea, repetitive vomiting, diarrhea, and stomachache. A family of isolectins (B. venenatus lectins, BVLs) was isolated as the toxic principles from the mushroom by successive 80% ammonium sulfate-precipitation, Super Q anion-exchange chromatography, and TSK-gel G3000SW gel filtration. Although BVLs showed a single band on SDS-PAGE, they were further divided into eight isolectins (BVL-1 to -8) by BioAssist Q anion-exchange chromatography. All the isolectins showed lectin activity and had very similar molecular weights as detected by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) analysis. Among them, BVL-1 and -3 were further characterized with their complete amino acid sequences of 99 amino acids determined and found to be identical to each other. In the hemagglutination inhibition assay, both proteins failed to bind to any mono- or oligo-saccharides tested and showed the same sugar-binding specificity to glycoproteins. Among the glycoproteins examined, asialo-fetuin was the strongest inhibitor. The sugar-binding specificity of each isolectin was also analyzed by using frontal affinity chromatography and surface plasmon resonance analysis, indicating that they recognized N-linked sugar chains, especially Galbeta1-->4GlcNAcbeta1-->4Manbeta1-->4GlcNAcbeta1-->4GlcNAc (Type II) residues in N-linked sugar chains. BVLs ingestion resulted in fatal toxicity in mice upon intraperitoneal administration and caused diarrhea upon oral administration in rats.","subitem_description_type":"Abstract"}]},"item_26_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_26_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"20096904","subitem_relation_type_select":"PMID"}}]},"item_26_relation_28":{"attribute_name":"出版者DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.phytochem.2009.12.003","subitem_relation_type_select":"DOI"}}]},"item_26_relation_34":{"attribute_name":"出版者版","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.phytochem.2009.12.003","subitem_relation_type_select":"URI"}}]},"item_26_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2009 Elsevier Ltd All rights reserved."}]},"item_26_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00319422","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18733700","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00774558","subitem_source_identifier_type":"NCID"}]},"item_26_subject_8":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_26_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Horibe, Masashi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kobayashi, Yuka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dohra, Hideo"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Morita, Tatsuya"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Murata, Takeomi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Usui, Taichi"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Nakamura-Tsuruta, Sachiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kamei, Masugu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hirabayashi, Jun"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Matsuura, Masanori"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamada, Mina"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Saikawa, Yoko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Hashimoto, Kimiko"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nakata, Masaya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kawagishi, Hirokazu"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-14"}],"displaytype":"detail","filename":"100630001.pdf","filesize":[{"value":"795.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"100630001.pdf","url":"https://shizuoka.repo.nii.ac.jp/record/4667/files/100630001.pdf"},"version_id":"4c2d2069-6404-4226-b2fb-ae8208e912d3"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Toxic isolectins from the mushroom Boletus venenatus","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Toxic isolectins from the mushroom Boletus venenatus","subitem_title_language":"en"}]},"item_type_id":"26","owner":"3","path":["222"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-06-30"},"publish_date":"2010-06-30","publish_status":"0","recid":"4667","relation_version_is_last":true,"title":["Toxic isolectins from the mushroom Boletus venenatus"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-02-14T02:48:24.406423+00:00"}