{"created":"2023-07-25T10:33:17.992897+00:00","id":4672,"links":{},"metadata":{"_buckets":{"deposit":"827286b7-dfaf-459f-aa72-2f2e5cf88869"},"_deposit":{"created_by":3,"id":"4672","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4672"},"status":"published"},"_oai":{"id":"oai:shizuoka.repo.nii.ac.jp:00004672","sets":["80:222"]},"author_link":["4526","2910","2907","2911","2924","2909","2925"],"item_26_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009-01-01","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"1","bibliographicPageEnd":"114","bibliographicPageStart":"108","bibliographicVolumeNumber":"139","bibliographic_titles":[{"bibliographic_title":"Journal of Biotechnology"}]}]},"item_26_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_26_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"A Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid expressing heavy and light chains of human 29IJ6 IgG was constructed and used to secrete recombinant antibody into silkworm larval hemolymph. Fifth instar silkworm larvae were reared and injected into the dorsum of the larvae with recombinant cysteine protease- and chitinase-deficient BmNPV (BmNPV-CP(-)-Chi(-)) bacmid/29IJ6 IgG and harvested after approximately 6 days. The total yield of recombinant 29IJ6 IgG was 36 microg/larvae, which is equivalent to 8 mg/kg of larvae. The recombinant antibody was purified to homogeneity using a HiTrap rProtein A FF column with a purification yield of 83.1%. The purified protein was identified by Western blot and ELISA experiments. The N-linked glycan structure of the purified protein was determined by the HPLC mapping method. The N-glycans of the 29IJ6 IgG glycoprotein produced in, and secreted by the silkworm larvae were composed exclusively of two kinds of paucimannose-type oligosaccharides, Manalpha1-6Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc and Manalpha1-6(Manalpha1-3)Manbeta1-4GlcNAcbeta1-4(Fucalpha1-6)GlcNAc.","subitem_description_type":"Abstract"}]},"item_26_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_26_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"18984019","subitem_relation_type_select":"PMID"}}]},"item_26_relation_28":{"attribute_name":"出版者DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jbiotec.2008.09.013","subitem_relation_type_select":"DOI"}}]},"item_26_relation_34":{"attribute_name":"出版者版","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"https://doi.org/10.1016/j.jbiotec.2008.09.013","subitem_relation_type_select":"URI"}}]},"item_26_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2008 Elsevier B.V. All rights reserved."}]},"item_26_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01681656","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA10458361","subitem_source_identifier_type":"NCID"}]},"item_26_subject_8":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"465","subitem_subject_scheme":"NDC"}]},"item_26_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Park, Enoch Y."}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Ishikiriyama, Motoki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishina, Takuya"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Tatsuya"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yagi, Hirokazu"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Koichi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Ueda, Hiroshi"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-14"}],"displaytype":"detail","filename":"100702005.pdf","filesize":[{"value":"569.2 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"100702005.pdf","url":"https://shizuoka.repo.nii.ac.jp/record/4672/files/100702005.pdf"},"version_id":"39ddf97b-aefd-46dc-831a-ceba35a8bace"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Animals","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Blotting, Western","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Bombyx","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Carbohydrate Conformation","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Cloning, Molecular","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Hemolymph","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Humans","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Immunoglobulin G","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Larva","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Lectins","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Nucleopolyhedrovirus","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Polysaccharides","subitem_subject_language":"en","subitem_subject_scheme":"Other"},{"subitem_subject":"Recombinant Proteins","subitem_subject_language":"en","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure","subitem_title_language":"en"}]},"item_type_id":"26","owner":"3","path":["222"],"pubdate":{"attribute_name":"公開日","attribute_value":"2010-07-02"},"publish_date":"2010-07-02","publish_status":"0","recid":"4672","relation_version_is_last":true,"title":["Human IgG1 expression in silkworm larval hemolymph using BmNPV bacmids and its N-linked glycan structure"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-02-14T02:53:08.325305+00:00"}