{"created":"2023-07-25T10:33:29.437579+00:00","id":4874,"links":{},"metadata":{"_buckets":{"deposit":"7583598f-b33d-43d4-a8cd-c77a8f36cf8f"},"_deposit":{"created_by":3,"id":"4874","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"4874"},"status":"published"},"_oai":{"id":"oai:shizuoka.repo.nii.ac.jp:00004874","sets":["129:395"]},"author_link":["5005","2669","5004","1503","5003","2665"],"item_26_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2009","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"1798","bibliographicPageStart":"1793","bibliographicVolumeNumber":"73","bibliographic_titles":[{"bibliographic_title":"Bioscience, Biotechnology, and Biochemistry"}]}]},"item_26_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_26_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Two genes, encoding YK-LiP1 and YK-LiP2, were cloned from the white-rot fungus Phanerochaete sordida YK-624, and a homologous expression system for the gene was constructed. Two full-length cDNAs (ylpA and ylpB) were isolated by degenerate RT-PCR and RACE-PCR. The results of N-terminal amino acid sequence analysis of native YK-LiP1 and YK-LiP2 showed that ylpA and ylpB coded for YK-LiP2 and YK-LiP1 respectively. The promoter of glyceraldehyde-3-phosphate dehydrogenase cloned from P. sordida YK-624 (PsGPD) was used to drive the expression of ylpA. Expression vector pGPD-g-ylpA was transformed into a P. sordida YK-624 uracil auxotrophic mutant, UV-64. The YlpA protein was secreted in active form by the transformants after 4 d of growth in a medium containing an excessive nitrogen source, whereas endogenous YK-LiP1 and YK-LiP2 were not produced. The physical and catalytic properties of the purified YlpA protein were very similar to those of YK-LiP2. These results suggest that homologous expression of recombinant YK-LiP2 was successful.","subitem_description_type":"Abstract"}]},"item_26_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Japan Society for Bioscience, Biotechnology, and Agrochemistry"}]},"item_26_relation_22":{"attribute_name":"NII論文ID","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10027544518","subitem_relation_type_select":"NAID"}}]},"item_26_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"19661691","subitem_relation_type_select":"PMID"}}]},"item_26_relation_28":{"attribute_name":"出版者DOI","attribute_value_mlt":[{"subitem_relation_type":"isIdenticalTo","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1271/bbb.90152","subitem_relation_type_select":"DOI"}}]},"item_26_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"09168451","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"13476947","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA10824164","subitem_source_identifier_type":"NCID"}]},"item_26_subject_8":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"658","subitem_subject_scheme":"NDC"}]},"item_26_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_970fb48d4fbd8a85","subitem_version_type":"VoR"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sugiura, Tatsuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yamagishi, Kenji"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kimura, Toshiyuki"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Nishida, Tomoaki"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kawagishi, Hirokazu"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Hirai, Hirofumi"}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-14"}],"displaytype":"detail","filename":"110517001.pdf","filesize":[{"value":"127.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110517001.pdf","url":"https://shizuoka.repo.nii.ac.jp/record/4874/files/110517001.pdf"},"version_id":"bbf29f33-6454-4de4-8834-8c75420f6426"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624","subitem_title_language":"en"}]},"item_type_id":"26","owner":"3","path":["395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-05-17"},"publish_date":"2011-05-17","publish_status":"0","recid":"4874","relation_version_is_last":true,"title":["Cloning and Homologous Expression of Novel Lignin Peroxidase Genes in the White-Rot Fungus Phanerochaete sordida YK-624"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-02-14T02:34:04.101940+00:00"}