{"created":"2023-07-25T10:34:11.941726+00:00","id":5726,"links":{},"metadata":{"_buckets":{"deposit":"eb3cd519-9637-413a-a215-8c351440bddc"},"_deposit":{"created_by":3,"id":"5726","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5726"},"status":"published"},"_oai":{"id":"oai:shizuoka.repo.nii.ac.jp:00005726","sets":["80:222"]},"author_link":["2910","5433","2907","2982","3113","3109","5434"],"item_26_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-11","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"152","bibliographicPageStart":"147","bibliographicVolumeNumber":"177","bibliographic_titles":[{"bibliographic_title":"Journal of Virological Methods"}]}]},"item_26_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_26_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Rous sarcoma virus group antigen protein-based virus-like particles (VLPs) are well known for their structural integrity and ease of handling. VLPs play an important role in drug delivery systems because they can be manipulated with ease. In this study, a new method was established for expressing Rous sarcoma virus group antigen protein based VLPs in silkworm larvae and establishing stably expressing insect cell lines. These VLPs have been isolated by ultracentrifugation using a sucrose step gradient of 10-60% (v/v), and their spherical structure has been confirmed using transmission electron microscopy (TEM). The spherical morphology is similar in both the silkworm larvae and in stably expressing cell lines. Silkworm larvae are better suited for producing Rous sarcoma virus group antigen protein-based VLPs on a large scale; yields from silkworm larvae were approximately 8.2-fold higher than yields from stable cell lines. These VLPs provide a new method for large-scale application in vaccine development and drug delivery systems.","subitem_description_type":"Abstract"}]},"item_26_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Elsevier"}]},"item_26_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21816175","subitem_relation_type_select":"PMID"}}]},"item_26_relation_28":{"attribute_name":"出版者DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1016/j.jviromet.2011.07.012","subitem_relation_type_select":"DOI"}}]},"item_26_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"Copyright © 2011 Published by Elsevier B.V."}]},"item_26_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"01660934","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"18790984","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA00259584","subitem_source_identifier_type":"NCID"}]},"item_26_subject_8":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_26_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Deo, Vipin Kumar"}],"nameIdentifiers":[{},{},{}]},{"creatorNames":[{"creatorName":"Tsuji, Yoshitaka"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Yasuda, Tomomi"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Kato, Tatsuya"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Sakamoto, Naonori"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Suzuki, Hisao"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Park, Enoch Y."}],"nameIdentifiers":[{},{},{},{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-15"}],"displaytype":"detail","filename":"110811001.pdf","filesize":[{"value":"701.1 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"110811001.pdf","url":"https://shizuoka.repo.nii.ac.jp/record/5726/files/110811001.pdf"},"version_id":"11318187-f36c-4dec-983c-7fb9fa92be5f"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae","subitem_title_language":"en"}]},"item_type_id":"26","owner":"3","path":["222"],"pubdate":{"attribute_name":"公開日","attribute_value":"2011-08-11"},"publish_date":"2011-08-11","publish_status":"0","recid":"5726","relation_version_is_last":true,"title":["Expression of an RSV-gag virus-like particle in insect cell lines and silkworm larvae"],"weko_creator_id":"3","weko_shared_id":3},"updated":"2024-02-14T02:51:55.940822+00:00"}