{"created":"2023-07-25T10:34:15.919717+00:00","id":5809,"links":{},"metadata":{"_buckets":{"deposit":"6b1d1807-60d8-47bf-8fca-7f19b80577f0"},"_deposit":{"created_by":3,"id":"5809","owners":[3],"pid":{"revision_id":0,"type":"depid","value":"5809"},"status":"published"},"_oai":{"id":"oai:shizuoka.repo.nii.ac.jp:00005809","sets":["129:395"]},"author_link":["1677","3463","5586","5587"],"item_26_biblio_info_5":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2011-06-03","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"2","bibliographicPageEnd":"276","bibliographicPageStart":"267","bibliographicVolumeNumber":"142","bibliographic_titles":[{"bibliographic_title":"Reproduction"}]}]},"item_26_description_30":{"attribute_name":"フォーマット","attribute_value_mlt":[{"subitem_description":"application/pdf","subitem_description_type":"Other"}]},"item_26_description_9":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"An antibody library against quail sperm plasma membrane components was established and a mAb, which strongly inhibits sperm perforations of the perivitelline membrane (PVM) was obtained from the library. The antigen molecule of the mAb showed an apparent molecular weight of 45  kDa, and was distributed both on the surface and in the acrosomal matrix of the sperm head. Periodate oxidation revealed that the epitope of the antigen includes a sugar moiety. Tandem mass spectrometry analysis of the antigen revealed that the mAb recognizes sperm acrosin. When sodium dodecyl sulfate-solubilized PVM immobilized on a polyvinylidene difluoride membrane was incubated with sperm plasma membrane lysates, the sperm acrosin was detected on the PVM immobilized on the membrane, indicating that the sperm acrosin interacts with the components of PVM. Indeed, the mAb effectively inhibited the binding of acrosome-intact sperm to the PVM. These results indicate that the 45  kDa sperm acrosin is involved in the binding of sperm to the PVM in fertilization of Japanese quail.","subitem_description_type":"Abstract"}]},"item_26_publisher_6":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"Society for Reproduction and Fertility"}]},"item_26_relation_26":{"attribute_name":"PubMed番号","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"21642383","subitem_relation_type_select":"PMID"}}]},"item_26_relation_28":{"attribute_name":"出版者DOI","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"10.1530/REP-11-0120","subitem_relation_type_select":"DOI"}}]},"item_26_rights_7":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"© 2011 Society for Reproduction and Fertility"}]},"item_26_source_id_19":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"14701626","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_20":{"attribute_name":"EISSN","attribute_value_mlt":[{"subitem_source_identifier":"17417899","subitem_source_identifier_type":"ISSN"}]},"item_26_source_id_23":{"attribute_name":"NII書誌ID","attribute_value_mlt":[{"subitem_source_identifier":"AA11501251","subitem_source_identifier_type":"NCID"}]},"item_26_subject_8":{"attribute_name":"NDC","attribute_value_mlt":[{"subitem_subject":"464","subitem_subject_scheme":"NDC"}]},"item_26_version_type_32":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_version_resource":"http://purl.org/coar/version/c_ab4af688f83e57aa","subitem_version_type":"AM"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Sasanami, Tomohiro"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Yoshizaki, Norio"}],"nameIdentifiers":[{}]},{"creatorNames":[{"creatorName":"Dohra, Hideo"}],"nameIdentifiers":[{},{},{},{}]},{"creatorNames":[{"creatorName":"Kubo, Hideo"}],"nameIdentifiers":[{}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2017-12-15"}],"displaytype":"detail","filename":"120410002.pdf","filesize":[{"value":"190.4 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"120410002.pdf","url":"https://shizuoka.repo.nii.ac.jp/record/5809/files/120410002.pdf"},"version_id":"9d71ed1a-c33f-4a5b-a171-34bc09d597e2"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"journal article","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica)","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica)","subitem_title_language":"en"}]},"item_type_id":"26","owner":"3","path":["395"],"pubdate":{"attribute_name":"公開日","attribute_value":"2012-04-10"},"publish_date":"2012-04-10","publish_status":"0","recid":"5809","relation_version_is_last":true,"title":["Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica)"],"weko_creator_id":"3","weko_shared_id":-1},"updated":"2024-02-14T02:34:06.485808+00:00"}