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Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica)
http://hdl.handle.net/10297/6548
http://hdl.handle.net/10297/65483cfa49e4-2ee7-4c42-8fac-53948ae6a033
名前 / ファイル | ライセンス | アクション |
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120410002.pdf (190.4 kB)
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Item type | 学術雑誌論文 / Journal Article_01(1) | |||||||||
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公開日 | 2012-04-10 | |||||||||
タイトル | ||||||||||
言語 | en | |||||||||
タイトル | Sperm acrosin is responsible for the sperm binding to the egg envelope during fertilization in Japanese quail (Coturnix japonica) | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
資源タイプ | ||||||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||||||
資源タイプ | journal article | |||||||||
著者 |
Sasanami, Tomohiro
× Sasanami, Tomohiro
WEKO
3463
× Yoshizaki, Norio× Dohra, Hideo
WEKO
1677
× Kubo, Hideo |
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書誌情報 |
Reproduction 巻 142, 号 2, p. 267-276, 発行日 2011-06-03 |
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出版者 | ||||||||||
出版者 | Society for Reproduction and Fertility | |||||||||
権利情報 | ||||||||||
権利情報 | © 2011 Society for Reproduction and Fertility | |||||||||
NDC | ||||||||||
主題Scheme | NDC | |||||||||
主題 | 464 | |||||||||
抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | An antibody library against quail sperm plasma membrane components was established and a mAb, which strongly inhibits sperm perforations of the perivitelline membrane (PVM) was obtained from the library. The antigen molecule of the mAb showed an apparent molecular weight of 45 kDa, and was distributed both on the surface and in the acrosomal matrix of the sperm head. Periodate oxidation revealed that the epitope of the antigen includes a sugar moiety. Tandem mass spectrometry analysis of the antigen revealed that the mAb recognizes sperm acrosin. When sodium dodecyl sulfate-solubilized PVM immobilized on a polyvinylidene difluoride membrane was incubated with sperm plasma membrane lysates, the sperm acrosin was detected on the PVM immobilized on the membrane, indicating that the sperm acrosin interacts with the components of PVM. Indeed, the mAb effectively inhibited the binding of acrosome-intact sperm to the PVM. These results indicate that the 45 kDa sperm acrosin is involved in the binding of sperm to the PVM in fertilization of Japanese quail. | |||||||||
ISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 14701626 | |||||||||
EISSN | ||||||||||
収録物識別子タイプ | ISSN | |||||||||
収録物識別子 | 17417899 | |||||||||
NII書誌ID | ||||||||||
収録物識別子タイプ | NCID | |||||||||
収録物識別子 | AA11501251 | |||||||||
PubMed番号 | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | PMID | |||||||||
関連識別子 | 21642383 | |||||||||
出版者DOI | ||||||||||
関連タイプ | isVersionOf | |||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | 10.1530/REP-11-0120 | |||||||||
フォーマット | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | application/pdf | |||||||||
著者版フラグ | ||||||||||
出版タイプ | AM | |||||||||
出版タイプResource | http://purl.org/coar/version/c_ab4af688f83e57aa |